ZEB1 anticorps (Internal Region)

N° du produit ABIN335118

Cet anticorps anti-ZEB1 est un anticorps Chèvre Polyclonal détectant ZEB1 dans WB, ELISA et IF. Adapté pour Humain et Souris.

Aperçu rapide pour ZEB1 anticorps (Internal Region) (ABIN335118)

Antigène: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ZEB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ZEB1 anticorps

Épitope: Internal Region

Fonction: AREB6 / ZEB1

Séquence: RPQIRQKIEN KPLQE

Specificité: This antibody is expected to recognize both reported isoforms (NP_001121600.1, NP_110378.3).

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1, NP_110378.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx.100 kDa band observed in Mouse Heart lysates (calculated MW of 122 kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ZEB1

Antigène: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Autre désignation: ZEB1

Sujet: ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding prote

ID gène: 6935, 21417, 25705

NCBI Accession: NP_001121600, NP_110378

Pathways: Regulation of Muscle Cell Differentiation