Cytokeratin 18 anticorps

N° du produit ABIN335330

Détail du produit anti-Cytokeratin 18 anticorps

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain, Souris, Rat, Chien, Porc, Hamster, Lapin, Poisson zèbre (Danio rerio), Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, mouse, rat, hamster, rabbit, chicken, canine and swine.

Purification: Purified

Immunogène: RGE53 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of cells.

Clone: RGE53

Isotype: IgG1

Information d'application

Indications d'application: RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. RGE53 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Cytokeratin 18 (ABIN335330)

van Leenders, Dijkman, Hulsbergen-van de Kaa, Ruiter, Schalken: "Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 8, pp. 1251-8, (2000) (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." dans: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, (1992) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, (1990) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Puts, Moesker, Kenemans, Vooijs, Ramaekers: "Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix." dans: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 4, Issue 4, pp. 300-13, (1986) (PubMed).

Ramaekers, Puts, Moesker, Kant, Huysmans, Haag, Jap, Herman, Vooijs: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." dans: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, (1983) (PubMed).

Ramaekers, Huysmans, Moesker, Kant, Jap, Herman, Vooijs: "Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 49, Issue 3, pp. 353-61, (1983) (PubMed).

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18 / Keratin K18 (KRT18 Produits)

Synonymes: anticorps CK18, anticorps K18, anticorps Krt1-18, anticorps CYK18, anticorps DreK18, anticorps cb83, anticorps dapk1, anticorps sb:cb83, anticorps wu:fa13f02, anticorps wu:fb36b04, anticorps krt18, anticorps MGC64569, anticorps MGC75922, anticorps KRT18, anticorps CK-18-A, anticorps K18-A, anticorps NC-11, anticorps cyk18, anticorps k18, anticorps krt18-a, anticorps krt18-b, anticorps krt18a, anticorps xkendob, anticorps keratin 18, anticorps keratin 18, type I L homeolog, anticorps keratin 18, type I, anticorps keratin, type I cytoskeletal 18, anticorps K18, simple type I keratin, anticorps Keratin, type I cytoskeletal 18, anticorps keratin 18, type I S homeolog, anticorps Krt18, anticorps KRT18, anticorps krt18, anticorps krt18.L, anticorps LOC700569, anticorps LOC100136778, anticorps k1c18, anticorps LOC100008885, anticorps krt18.S

Sujet: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Pathways: Apoptose, Caspase Cascade in Apoptosis