Keratin 10/13 anticorps

N° du produit ABIN336868

Cet anticorps Souris Monoclonal détecte spécifiquement Keratin 10/13 dans IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Keratin 10/13 anticorps (ABIN336868)

Antigène: Keratin 10/13

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC)

Clone: CK208

Détail du produit

Specificité: Molecular Weight: CK 10 is 56.9 kDa and CK 13 is 53 kDa.

Immunogène: Cytokeratin extraction from human ectocervical epithelium

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry (IHC), Immunocytochemistry (ICC) (frozen tissue sections ONLY or cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil or skin. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The concentrated antibodies are in supplied in PBS with 1% BSA, 0.05% azide, pH 7.4.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détail du antigène

Antigène: Keratin 10/13

Autre désignation: Cytokeratin 10+13

Sujet: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.