Cytokeratin 18 anticorps (FITC)
Aperçu rapide pour Cytokeratin 18 anticorps (FITC) (ABIN336873)
Antigène
Voir toutes Cytokeratin 18 (KRT18) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- Molecular Weight: 45 kDa.
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Immunogène
- Human breast cancer PMC 42 cells
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Isotype
- IgG1 kappa
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Indications d'application
- IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears). For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (Antigen retriever like citrate enhances the staining of FFPE tissue sections) (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Human skin, breast, lung ca.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Cytokeratins are intermediate filament keratins found in
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Autre désignation
- Cytokeratin 18
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Pathways
- Apoptose, Caspase Cascade in Apoptosis
Antigène
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