POLL anticorps (AA 563-575)

N° du produit ABIN337014

Cet anticorps anti-POLL Polyclonal Chèvre (ABIN337014) détecte spécifiquement POLL dans ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Lapin, Boeuf (Vache), Porc, Roussette (Chauve-souris), Hamster et Singe.

Aperçu rapide pour POLL anticorps (AA 563-575) (ABIN337014)

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivité: Humain, Rat, Souris, Lapin, Boeuf (Vache), Porc, Roussette (Chauve-souris), Hamster, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POLL est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLL anticorps

Épitope: AA 563-575

Specificité: Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%) Dog, Opossum, Turkey, Chicken (92%) Xenopus, Zebrafish, Sea anemone (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%), Dog, Opossum, Turkey, Chicken (92%), Xenopus, Zebrafish, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur POLL

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Autre désignation: POLL / DNA Polymerase Lambda

Sujet: Name/Gene ID: POLL

Synonyms: POLL, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase lambda, DNA polymerase kappa, Pol beta2, BETAN, Pol Lambda, POLKAPPA

ID gène: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response