Prohibitin 2 anticorps (AA 88-299)

N° du produit ABIN337044

Cet anticorps anti-Prohibitin 2 Polyclonal Lapin (ABIN337044) détecte spécifiquement Prohibitin 2 dans WB, IHC, IHC (p), IP et ICC. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour Prohibitin 2 anticorps (AA 88-299) (ABIN337044)

Antigène: Prohibitin 2 (PHB2)

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Prohibitin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-Prohibitin 2 anticorps

Épitope: AA 88-299

Specificité: Recognizes mouse REA, Mr 37kD. Species cross-reactivity: human and rat.

Purification: Protein A purified

Immunogène: His6-tagged fusion protein corresponding to aa88-299 of human REA-1. The immunizing sequence is identical in mouse.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunocytochemistry. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates REA from MCF-7 and 3T3 whole cell extracts. Immunocytochemistry: Nuclear staining of REA in paraformaldehyde fixed, Triton X-100 permeabilized MCF-7 cells.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Prohibitin 2

Antigène: Prohibitin 2 (PHB2)

Autre désignation: Prohibitin 2 / PHB2

Sujet: Name/Gene ID: PHB2
Family: Transcription factor

Synonyms: PHB2, BCAP37, BAP, D-prohibitin, p22, Prohibitin-2, B-cell associated protein, Bap37, PNAS-141, Prohibitin 2, REA

ID gène: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling