Occludin anticorps (C-Term)

N° du produit ABIN337048

Cet anticorps Souris Monoclonal détecte spécifiquement Occludin dans WB, IHC, ELISA, IF et IHC (p). Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 1 publication.

Aperçu rapide pour Occludin anticorps (C-Term) (ABIN337048)

Antigène: Occludin (OCLN)

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Occludin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OC-3F10

Détail du produit anti-Occludin anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.

Purification: Protein A purified

Immunogène: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Références pour anticorps anti-Occludin (ABIN337048)

Krueger, Härtig, Reichenbach, Bechmann, Michalski: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." dans: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: OCLN / Occludin

Sujet: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

ID gène: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C