P4HB anticorps

N° du produit ABIN337100

L’anticorps anti-P4HB Polyclonal Lapin est utilisé pour la détection de P4HB dans des échantillons de Humain. Il a été validé pour WB, IHC, ICC, IF et IHC (p).

Aperçu rapide pour P4HB anticorps (ABIN337100)

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp P4HB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-P4HB anticorps

Specificité: Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ICC (1:50), IF (1:50), IHC, IHC-P (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur P4HB

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: PDI / P4HB

Sujet: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

ID gène: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism