APOE anticorps (AA 305-317)

N° du produit ABIN337117

Cet anticorps Chèvre Polyclonal détecte spécifiquement APOE dans WB, IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour APOE anticorps (AA 305-317) (ABIN337117)

Antigène: APOE (Apolipoprotein E (APOE))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-APOE anticorps

Épitope: AA 305-317

Specificité: Human APOE / Apolipoprotein E.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Gibbon (100%) Chimpanzee, Gorilla, Marmoset (92%) Baboon (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-VGTSAAPVPSDNH from the C-terminus of human APOE / Apolipoprotein E (NP_000032.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon (100%), Chimpanzee, Gorilla, Marmoset (92%), Baboon (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur APOE

Antigène: APOE (Apolipoprotein E (APOE))

Autre désignation: APOE / Apolipoprotein E

Sujet: Name/Gene ID: APOE

Synonyms: APOE, AD2, Apo-E, Apolipoprotein E3, Apolipoprotein E, LDLCQ5, LPG

ID gène: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism