Iba1 anticorps (AA 138-148)

N° du produit ABIN337130

L’anticorps anti-Iba1 Polyclonal Chèvre est utilisé pour la détection de Iba1 dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA et IHC (p).

Aperçu rapide pour Iba1 anticorps (AA 138-148) (ABIN337130)

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Iba1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Iba1 anticorps

Épitope: AA 138-148

Specificité: Human AIF1 / IBA1. This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (91%) Goat, Platypus, Slime mold, Spikemoss, Chaetomium (82%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-YEEKAREKEKP from the C-terminus of human AIF1 / IBA1 (NP_116573.1, NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (91%), Goat, Platypus, Slime mold, Spikemoss, Chaetomium (82%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (1:16000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Iba1

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: AIF1 / IBA1

Sujet: Name/Gene ID: AIF1

Synonyms: AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1

ID gène: 199

NCBI Accession: NP_116573, NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration