CK1 epsilon anticorps (AA 403-416)

N° du produit ABIN337139

L’anticorps anti-CK1 epsilon Polyclonal Chèvre est utilisé pour la détection de CK1 epsilon dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour CK1 epsilon anticorps (AA 403-416) (ABIN337139)

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CK1 epsilon est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CK1 epsilon anticorps

Épitope: AA 403-416

Specificité: Human CSNK1E. Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%) Xenopus (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-PASQTSVPFDHLGK from the C-terminus of human CSNK1E (NP_001885.1, NP_689407.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%), Xenopus (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CK1 epsilon

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Autre désignation: CSNK1E / CK1 Epsilon

Sujet: Name/Gene ID: CSNK1E
Subfamily: Casein kinase I
Family: Protein Kinase

Synonyms: CSNK1E, Casein kinase 1 epsilon, Casein kinase i epsilon, CK1 epsilon, CKIE, CKIepsilon, CKI-epsilon, HCKIE, Casein kinase 1, epsilon

ID gène: 1454

NCBI Accession: NP_001885, NP_689407

UniProt: P49674

Pathways: Signalisation Hedgehog, M Phase