CDK2 anticorps

N° du produit ABIN337208

L’anticorps Souris Monoclonal anti-CDK2 a été validé pour WB, IHC, ELISA, IP, IF et IHC (p). Il convient pour détecter CDK2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CDK2 anticorps (ABIN337208)

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CDK2 anticorps

Specificité: Recognizes human Cdk2. Species cross-reactivity: mouse and rat.

Purification: Protein A purified

Immunogène: Human recombinant cdk2 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a, IgG2b

Information d'application

Indications d'application: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 2 μg/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 μg/mL for 2hrs at RT. Positive control: HeLa cells.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur CDK2

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Autre désignation: CDK2

Sujet: Name/Gene ID: CDK2
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK2, Cell division protein kinase 2, CDC2-related protein kinase, p33 protein kinase, p33(CDK2), CDKN2, Cyclin-dependent kinase 2

ID gène: 1017

UniProt: P24941

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA