CD35 anticorps

N° du produit ABIN337278

Cet anticorps anti-CD35 Monoclonal Souris (Clone E11) (ABIN337278) détecte spécifiquement CD35 dans FACS, IHC, WB, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Babouin, Cynomolgus et Macaque rhésus.

Aperçu rapide pour CD35 anticorps (ABIN337278)

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivité: Humain, Babouin, Cynomolgus, Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD35 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: E11

Détail du produit anti-CD35 anticorps

Specificité: Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.

Purification: Protein A purified

Immunogène: Human acute monocytic leukaemia cells.

Type of Immunogen: Cells

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the diluted antibody to label 10^6 cells or 100 μL whole blood. Histology Positive Control Tissue: Tonsil.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in TCS.

Agent conservateur: Azide free

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur CD35

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: CR1 / CD35

Sujet: Name/Gene ID: CR1
Subfamily: Serine S1
Family: Protease

Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

ID gène: 1378

UniProt: P17927

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity