MMP2 anticorps (AA 468-483)

N° du produit ABIN337360

Cet anticorps Souris Monoclonal détecte spécifiquement MMP2 dans WB, IHC, ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Humain et Porc.

Aperçu rapide pour MMP2 anticorps (AA 468-483) (ABIN337360)

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MMP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MMP2 anticorps

Épitope: AA 468-483

Marque: IHC-plus™

Specificité: Recognizes the precursor and active forms of human MMP-2. Species cross-reactivity: mouse, rat, rabbit, porcine, bovine and equine MMP-2, but not with human MMP-1, 3, 9 and 13.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%) Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%) Bovine (88%) Chicken (81%).

Purification: Purified

Immunogène: Synthetic oligopeptide corresponding to aa residues 468-483 (VTPRDKPMGPLLVATF) of human MMP-2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%), Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%), Bovine (88%), Chicken (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr (0.9 μg/mL), IHC-P (10 μg/mL), WB (1 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 2 % BSA (protease free).

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur MMP2

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: MMP2 / MMP-2

Sujet: Name/Gene ID: MMP2
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP2, 72 kd type iv collagenase, 72 kDa gelatinase, 72 kd gelatinase, 72 kDa type IV collagenase, CLG4A, CLG4, GelA, Gelatinase A, Matrix metalloproteinase-2, MMP-2, MONA, Neutrophil gelatinase, TBE-1, Tbe-1 metalloprotease, Collagenase type IV-A, Matrix metalloproteinase-II, MMP-II

ID gène: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response