HSF1 anticorps

N° du produit ABIN337399

Cet anticorps Lapin Polyclonal détecte spécifiquement HSF1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Lapin et Singe.

Aperçu rapide pour HSF1 anticorps (ABIN337399)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Humain, Souris, Rat, Lapin, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HSF1 anticorps

Marque: IHC-plus™

Specificité: Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.

Purification: Protein A purified

Immunogène: Recombinant human HSF-1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Positive control: Recombinant Human HSF-1 Protein.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: HSF1

Sujet: Name/Gene ID: HSF1

Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1

ID gène: 3297

UniProt: Q00613