Ubiquitin anticorps

N° du produit ABIN340405

Cet anticorps Souris Monoclonal détecte spécifiquement Ubiquitin dans WB, IHC, IF, IHC (p), IP et IHC (fro). Il présente une réactivité envers Humain et Boeuf (Vache).

Aperçu rapide pour Ubiquitin anticorps (ABIN340405)

Antigène: Ubiquitin (Ub)

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ubiquitin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Ubiquitin anticorps

Specificité: Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.

Purification: Purified

Immunogène: Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)

Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (  pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing.

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway