Histone 3 anticorps (H3K36me3)

N° du produit ABIN3434046

Cité dans 5 publications. Cet anticorps anti-Histone 3 Polyclonal Lapin (ABIN3434046) détecte spécifiquement Histone 3 dans WB, ChIP, DB, ChIP-seq et CUT&Tag. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Histone 3 anticorps (H3K36me3) (ABIN3434046)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K36me3

Fonction: Histone H3K36me3 antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 10 μg per ChIP ChIP-Seq: 4 μg each WB: 0.5 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details, see Catalog No. 91265.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN3434046)

Powers, Parvanov, Baker, Walker, Petkov, Paigen: "The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo." dans: PLoS genetics, Vol. 12, Issue 6, pp. e1006146, (2016) (PubMed).

Ho, Nateras, Yan, Park, Jensen, Borges, Lee, Champion, Tibes, Bryce, Carballido, Todd, Joseph, Wong, Parker, Stanton, Castle: "A Multidisciplinary Biospecimen Bank of Renal Cell Carcinomas Compatible with Discovery Platforms at Mayo Clinic, Scottsdale, Arizona." dans: PLoS ONE, Vol. 10, Issue 7, pp. e0132831, (2015) (PubMed).

Pham, Inoue, Vu, Nguyen, Nakayashiki, Ikeda, Nakayashiki: "MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis." dans: PLoS genetics, Vol. 11, Issue 7, pp. e1005385, (2015) (PubMed).

Feng, Zhang, Shea, Creighton, Coarfa, Hilsenbeck, Lanz, He, Wang, Fu, Nardone, Song, Bradner, Mitsiades, Mitsiades, Osborne, Schiff, OMalley: "An epigenomic approach to therapy for tamoxifen-resistant breast cancer." dans: Cell research, Vol. 24, Issue 7, pp. 809-19, (2014) (PubMed).

Mews, Zee, Liu, Donahue, Garcia, Berger: "Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence." dans: Molecular and cellular biology, Vol. 34, Issue 21, pp. 3968-80, (2014) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522