CD34 anticorps

N° du produit ABIN343702

Cet anticorps Souris Monoclonal détecte spécifiquement CD34 dans FACS, WB, IHC (p), IP, IHC (fro) et Func. Il présente une réactivité envers Humain et Non-Human Primate et a été mentionné dans 6+ publications.

Aperçu rapide pour CD34 anticorps (ABIN343702)

Antigène: CD34

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: QBEnd-10

Détail du produit anti-CD34 anticorps

Fonction: Anti-Hu CD34 Purified Azide Free

Specificité: The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

Aucune reactivité croisée: Boeuf (Vache), Chien, Rat, Mouton

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Human endothelial vesicles

Isotype: IgG1

Information d'application

Indications d'application: Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: TF-1 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD34 (ABIN343702)

Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." dans: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).

Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, (1994) (PubMed).

Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." dans: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, (1992) (PubMed).

Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." dans: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, (1992) (PubMed).

Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." dans: Histopathology, Vol. 17, Issue 3, pp. 237-42, (1991) (PubMed).

Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, (1990) (PubMed).

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

ID gène: 947

UniProt: P28906