p130 anticorps

N° du produit ABIN349607

N° du produit (Fournisseur): 600-401-b11

Cet anticorps Lapin Polyclonal détecte spécifiquement p130 dans WB, ELISA et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour p130 anticorps (ABIN349607)

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p130 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-p130 anticorps

N° du produit (Fournisseur): 600-401-b11

Fournisseur: Rockland

Fonction: pRb2 p130 Antibody

Réactivité croisée (Details): This antiserum is directed against Spa310 and reacts with the Spa310 domain of pRb2/p130 from human tissues. Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.

Attributs du produit: Synonyms: rabbit anti-pRb2/p130 antibody, rabbit anti-pRb2 antibody, rabbit anti-p130 antibody, rabbit anti-Spa310 antibody, 130 kDa retinoblastoma associated protein antibody, RBR-2 antibody, RBL2 antibody, RB2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody

Purification: Antiserum

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein.  A residue of cysteine was added to facilitate coupling.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody is tested for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2 kDa in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:75,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.39 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur p130

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Autre désignation: RBL2

Sujet: Background: Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.

ID gène: 5934

UniProt: Q08999

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases