MYB anticorps

N° du produit ABIN349608

N° du produit (Fournisseur): 600-401-b06

Cet anticorps Lapin Polyclonal détecte spécifiquement MYB dans WB et ELISA. Il présente une réactivité envers Souris et Singe.

Aperçu rapide pour MYB anticorps (ABIN349608)

Antigène: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivité: Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYB est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-MYB anticorps

N° du produit (Fournisseur): 600-401-b06

Fournisseur: Rockland

Fonction: c-Myb Antibody

Réactivité croisée (Details): This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells.

Attributs du produit: Synonyms: rabbit anti-c-Myb Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of mouse c-Myb protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb.  Specific conditions for reactivity and detection of c-Myb should be optimized by the end user.  Expect a band approximately ~75 kDa in size corresponding to c-Myb by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,500

ELISA Dilution: 1:10,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MYB

Antigène: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Autre désignation: Myb

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.

ID gène: 17863, 110556654

UniProt: P06876

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process