LGR4 anticorps (Internal Region)

N° du produit ABIN349637

N° du produit (Fournisseur): 200-301-b45

Cet anticorps Souris Monoclonal détecte spécifiquement LGR4 dans ELISA, WB et IHC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour LGR4 anticorps (Internal Region) (ABIN349637)

Antigène: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LGR4 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 6G8-B3-G5-C3

Détail du produit anti-LGR4 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 200-301-b45

Fournisseur: Rockland

Fonction: LGR4 Antibody

Réactivité croisée (Details): This antibody is specific for human LGR4 protein.

Attributs du produit: Synonyms: mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4

Purification: Anti-LGR4 purified from concentrated tissue culture supernate by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Immunogen Type: Conjugated Peptide

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-LGR4 monoclonal antibody has been tested by ELISA and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.13 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-LGR4 (ABIN349637)

Yi, Xiong, Gong, Bellister, Ellis, Liu: "Analysis of LGR4 receptor distribution in human and mouse tissues." dans: PLoS ONE, Vol. 8, Issue 10, pp. e78144, (2014) (PubMed).

Détails sur LGR4

Antigène: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Autre désignation: LGR4

Sujet: Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

ID gène: 55366, 157694513

UniProt: Q8N537