GRP78 anticorps

N° du produit ABIN349665

Cité dans 2 publications. Cet anticorps anti-GRP78 Polyclonal Lapin (ABIN349665) détecte spécifiquement GRP78 dans WB, ELISA, IF et EM. L’anticorps est réactif avec des échantillons de Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radis, Rape et Schinus molle.

Aperçu rapide pour GRP78 anticorps (ABIN349665)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radis, Rape, Schinus molle

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP78 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)

Détail du produit anti-GRP78 anticorps

Fonction: Anti-BiP | Lumenal-binding protein (rabbit antibody)

Réactivité croisée (Details): Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Not reactive in: Ostreococcus tauri, Schizosaccharomyces pombe

Homologie: Arabis alpina,Capsella rubella,Capsicum annuum,Citrus clementina,Citrus sinsensis,Eucalyptus grandis,Glycine max,Hordeum vulgare,Isatis tincorina,Prunus persica,Triticum aestivium,Petunia hybrida,Picea sitcHensis,Populus trichocarpa,Ricinus comminus,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3

Isotype: IgG

Information d'application

Indications d'application: 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

Commentaires:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-GRP78 (ABIN349665)

Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." dans: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." dans: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: BiP

Sujet: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Poids moléculaire: 73.5 | 80 kDa

ID gène: 834207

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling