GRP78 anticorps

N° du produit ABIN349665

Cet anticorps anti-GRP78 est un anticorps Lapin Polyclonal détectant GRP78 dans WB, ELISA, IF et EM. Adapté pour Chlamydomonas, Olive et Zea mays subsp. parviglumis (Balsas teosinte). Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour GRP78 anticorps (ABIN349665)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Chlamydomonas, Olive, Zea mays subsp. parviglumis (Balsas teosinte)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP78 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)

Détail du produit anti-GRP78 anticorps

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 73.5 / 80 kDa

Purification: affinity purified

Immunogène: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At5g09080 Q8H1B3

Isotype: IgG

Information d'application

Indications d'application: Recommended Dilution: 1 : 8000 (ELISA), 1 : 2000 with standard ECL (WB), 1 : 600 ( IF).

Commentaires:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Conseil sur la manipulation: Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Stock: -20 °C

Références pour anticorps anti-GRP78 (ABIN349665)

Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." dans: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." dans: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: BiP

Sujet: AGI Code: At5g42020, At5g42020
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Poids moléculaire: expected: 73.5 kDa, apparent: 80 kDa

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling