PRNP anticorps (Prion)

N° du produit ABIN349704

Cet anticorps anti-PRNP est un anticorps Souris Monoclonal détectant PRNP dans WB. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour PRNP anticorps (Prion) (ABIN349704)

Antigène: PRNP (Prion Protein (PRNP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PRNP est non-conjugé

Application: Western Blotting (WB)

Clone: EM-20

Détail du produit anti-PRNP anticorps

Épitope: Prion

Fonction: Anti-Hu CD230 / Prion Purified

Specificité: The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant human prion protein

Isotype: IgG2a

Information d'application

Indications d'application: Western blotting: Recommended dilution: 0.5 μg/mL, non-reducing conditions are essential.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-PRNP (ABIN349704)

Dvorakova, Vranac, Janouskova, ?ernilec, Koren, Lukan, Nováková, Matej, Holada, ?urin Šerbec: "Detection of the GPI-anchorless prion protein fragment PrP226* in human brain." dans: BMC neurology, Vol. 13, pp. 126, (2013) (PubMed).

Détails sur PRNP

Antigène: PRNP (Prion Protein (PRNP))

Autre désignation: CD230

Sujet: Prion protein,CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.,Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC

ID gène: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation