Choline Acetyltransferase anticorps

N° du produit ABIN350211

Cet anticorps anti-Choline Acetyltransferase est un anticorps Mouton Polyclonal détectant Choline Acetyltransferase dans WB et IHC. Adapté pour Souris.

Aperçu rapide pour Choline Acetyltransferase anticorps (ABIN350211)

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Choline Acetyltransferase anticorps

Fonction: Sheep antibody to ChAT

Specificité: Specific for ChAT

Réactivité croisée: Humain, Marmoset, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: ChAT

Sujet: Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2), also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy negative tests for anti-AChR antibodies and abrupt episodic crises with increased weakness bulbar paralysis and apnea precipitated by undue exertion fever or excitement. Inheritance is autosomal recessive.

UniProt: P32738

Pathways: Skeletal Muscle Fiber Development