GAPDH anticorps

N° du produit ABIN350328

Cet anticorps anti-GAPDH est un anticorps Lapin Polyclonal détectant GAPDH dans WB et IHC. Adapté pour Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour GAPDH anticorps (ABIN350328)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GAPDH anticorps

Fonction: Rabbit antibody to GAPDH

Specificité: Specific for GAPDH.

Réactivité croisée: Humain, Marmoset, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 1-5,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-GAPDH (ABIN350328)

Gengenbacher, Müller-Rudorf, Poggio, Gräßel, Dumit, Kreutmair, Lippert, Duyster, Illert: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." dans: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

UniProt: P04797