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GAPDH anticorps (C-Term)

L’anticorps Mouton Polyclonal anti-GAPDH a été validé pour WB et IHC. Il convient pour détecter GAPDH dans des échantillons de Rat.
N° du produit ABIN350330

Aperçu rapide pour GAPDH anticorps (C-Term) (ABIN350330)

Antigène

Voir toutes GAPDH Anticorps
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité

  • 221
  • 152
  • 131
  • 65
  • 47
  • 41
  • 29
  • 26
  • 25
  • 20
  • 19
  • 19
  • 15
  • 15
  • 14
  • 13
  • 12
  • 9
  • 9
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Hôte

  • 168
  • 90
  • 16
  • 8
  • 4
Mouton

Clonalité

  • 185
  • 99
Polyclonal

Conjugué

  • 165
  • 31
  • 18
  • 16
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp GAPDH est non-conjugé

Application

  • 231
  • 101
  • 77
  • 75
  • 41
  • 37
  • 36
  • 33
  • 16
  • 15
  • 15
  • 15
  • 7
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Épitope

    • 35
    • 22
    • 13
    • 12
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Fonction

    Sheep antibody to GAPDH

    Specificité

    Specific for GAPDH.

     Réactivité croisée

    Humain, Souris, Rat

    Réactivité croisée (Details)

    Other species not yet tested.

    Purification

    IgG

    Immunogène

    A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

    Isotype

    IgG
  • Indications d'application

    IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

    Conseil sur la manipulation

    Avoid freeze and thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

    Date de péremption

    12 months
  • Antigène

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    Autre désignation

    GAPDH

    Sujet

    FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

    UniProt

    P04797
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