NOS1 anticorps (C-Term)

N° du produit ABIN350524

Cet anticorps Chèvre Polyclonal détecte spécifiquement NOS1 dans WB et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour NOS1 anticorps (C-Term) (ABIN350524)

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NOS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NOS1 anticorps

Épitope: C-Term

Fonction: Goat antibody to nNOS

Specificité: Specific for nNOS

Réactivité croisée: Cobaye, Humain, Souris, Lapin, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG purified. Sterilised UV-C irradiated acidified to pH 3.5 then neutralised

Immunogène: Synthetic peptides from the c-terminal region of human nNOS conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur NOS1

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Autre désignation: nNOS

Sujet: FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. CATALYTIC ACTIVITY: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+. ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP. SUBCELLULAR LOCATION: Cell membrane sarcolemma, Peripheral membrane protein. Cell projection dendritic spine. Note=In skeletal muscle it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons enriched in dendritic spines. TISSUE SPECIFICITY: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis urethra prostate and skeletal muscle and coexists with the cerebellar nnos in the pelvic plexus bladder and liver and is detectable in the cerebellum.

UniProt: P29476

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction