ERK1/2 anticorps (pThr202, pTyr204)

N° du produit ABIN350736

Cet anticorps anti-ERK1/2 est un anticorps Lapin Polyclonal détectant ERK1/2 dans WB et IHC. Adapté pour Humain.

Aperçu rapide pour ERK1/2 anticorps (pThr202, pTyr204) (ABIN350736)

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1/2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ERK1/2 anticorps

Épitope: pThr202, pTyr204

Fonction: Rabbit antibody to phospho T202 & Y204 Erk1 Erk2

Specificité: Specific for Erk1 and Erk2.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from the phospho T202 & Y204 of human Erk1 Erk2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish xenopus and chicken.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur ERK1/2

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Autre désignation: Erk1 Erk2

Sujet: FUNCTION: Involved in both the initiation and regulation of meiosis mitosis and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF. SUBUNIT: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.

UniProt: P27361