ERK1/2 anticorps
Aperçu rapide pour ERK1/2 anticorps (ABIN351562)
Antigène
Voir toutes ERK1/2 (MAPK1/3) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.
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Purification
- Immunoaffinity purified
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Immunogène
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Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Indications d'application
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Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P, IP (1:50), WB (1:1000)
Usage: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25. -
Commentaires
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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Autre désignation
- ERK1 + ERK2
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Sujet
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Name/Gene ID: ERK1/ERK2
Synonyms: ERK1/ERK2
This antibody detects: ERK1 + ERK2
Antigène
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