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ERK1/2 anticorps

Cet anticorps Lapin Polyclonal détecte spécifiquement ERK1/2 dans WB, ELISA, IHC, IHC (p), IF, FACS, IP et ICC. Il présente une réactivité envers Humain, Souris, Rat et Hamster.
N° du produit ABIN351562

Aperçu rapide pour ERK1/2 anticorps (ABIN351562)

Antigène

Voir toutes ERK1/2 (MAPK1/3) Anticorps
ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivité

  • 184
  • 161
  • 156
  • 21
  • 21
  • 18
  • 7
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat, Hamster

Hôte

  • 139
  • 48
  • 4
Lapin

Clonalité

  • 134
  • 57
Polyclonal

Conjugué

  • 84
  • 9
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Cet anticorp ERK1/2 est non-conjugé

Application

  • 139
  • 66
  • 51
  • 49
  • 43
  • 33
  • 24
  • 24
  • 20
  • 13
  • 6
  • 4
  • 4
  • 3
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Specificité

    Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.

    Purification

    Immunoaffinity purified

    Immunogène

    Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Indications d'application

    Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P, IP (1:50), WB (1:1000)

    Usage: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25.

    Commentaires

    Target Species of Antibody: Rat

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

    Autre désignation

    ERK1 + ERK2

    Sujet

    Name/Gene ID: ERK1/ERK2

    Synonyms: ERK1/ERK2

    This antibody detects: ERK1 + ERK2
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