BrdU anticorps (FITC)

N° du produit ABIN356283

L’anticorps Souris Monoclonal anti-BrdU a été validé pour FACS, IF et IHC (p). Il convient pour détecter BrdU dans des échantillons de .

Aperçu rapide pour BrdU anticorps (FITC) (ABIN356283)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BRDU206

Détail du produit anti-BrdU anticorps (FITC)

Immunogène: BrdU conjugated to KLH. Remarks: Epitope: Bromodeoxyuridine.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. For staining of FFPE tissue sections, incubate with 4 N HCl for 20 minutes at RT, followed bytreatment with proteolytic digestion for 10 minutes at RT. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Liver of experimental animal injected with BrdU or cell linegrown in presence of BrdU.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Classe de substances: Chemical

Sujet: Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside which is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific to BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA by heat or acid.