Ubiquitin anticorps

N° du produit ABIN356467

Cet anticorps Lapin Polyclonal détecte spécifiquement Ubiquitin dans IF, IHC (p) et IHC (fro). Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache) et Cheval.

Aperçu rapide pour Ubiquitin anticorps (ABIN356467)

Antigène: Ubiquitin (Ub)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ubiquitin est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Ubiquitin anticorps

Specificité: This antibody stains senile plaques and neurons.
Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.

Purification: Protein A Chromatography

Immunogène: Purified Human Ubiquitin.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigenretriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling atRT for 15-20 minutes. Immunofluorescence. Recommended Positive Control: Human brain from Alzheimer's disease.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 3 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Sujet: Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin, it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer's disease, lewy bodies in Parkinson's disease, pick bodies in Pick's disease, inclusions in motor neuron disease, Mallory's Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.Synonyms: Polyubiquitin-B, UBB

ID gène: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway