Histone 3 anticorps (H3K9me2)
Aperçu rapide pour Histone 3 anticorps (H3K9me2) (ABIN356586)
Antigène
Voir toutes Histone 3 (H3) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- H3K9me2
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Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Immunogène
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide methylated on K9(2 methyl groups) selected from aa 6~12 of human Histone 3.
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Isotype
- Ig Fraction
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Indications d'application
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ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Histone 3 (H3) (Histone H3 (H3))
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Autre désignation
- Histone H3
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Sujet
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Synonyms: H3 Histone family, HIST1H3
Antigène
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