UBE2I anticorps (N-Term)

N° du produit ABIN356594

Détail du produit anti-UBE2I anticorps

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Épitope: N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2I est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur UBE2I

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Autre désignation: UBC9 / UBE2I (UBE2I Produits)

Synonymes: anticorps C358B7.1, anticorps P18, anticorps UBC9, anticorps ubc9, anticorps ube2ia, anticorps zUbc9, anticorps 5830467E05Rik, anticorps F830028O17Rik, anticorps Ubce2i, anticorps Ubce9, anticorps UbcE2A, anticorps ubce9, anticorps T13J8.70, anticorps T13J8_70, anticorps UBIQUITIN-PROTEIN LIGASE, anticorps ubiquitin conjugating enzyme 9, anticorps ubiquitin conjugating enzyme E2 I, anticorps ubiquitin-conjugating enzyme E2Ib, anticorps ubiquitin-conjugating enzyme E2L, anticorps ubiquitin-conjugating enzyme E2I, anticorps ubiquitin conjugating enzyme E2I S homeolog, anticorps ubiquitin conjugating enzyme 9, anticorps SUMO-conjugating enzyme UBC9, anticorps UBE2I, anticorps ube2ib, anticorps UBE2L, anticorps Ube2i, anticorps ube2i.S, anticorps UBC9, anticorps ubc-9, anticorps LOC108703134

Sujet: UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I

ID gène: 7329, 9606

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway