CDC25B anticorps (pSer187)

N° du produit ABIN358100

Détail du produit anti-CDC25B anticorps

Antigène: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Épitope: pSer187

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC25B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects CDC25B pSer187.

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S187 of human Cdc25B.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur CDC25B

Antigène: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Autre désignation: CDC25B (CDC25B Produits)

Synonymes: anticorps CDC25B, anticorps AI604853, anticorps cell division cycle 25B, anticorps cdc25b, anticorps CDC25B, anticorps Cdc25b

Sujet: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.Synonyms: CDC25HU2, Dual specificity phosphatase Cdc25B, M-phase inducer phosphatase 2

Poids moléculaire: 64987 Da

ID gène: 994, 9606

UniProt: P30305

Pathways: Cycle Cellulaire, M Phase, Autophagy