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WEE1 anticorps (pSer123)

Cet anticorps anti-WEE1 est un anticorps Lapin Polyclonal détectant WEE1 dans IHC (p) et EIA. Adapté pour Humain.
N° du produit ABIN358251

Aperçu rapide pour WEE1 anticorps (pSer123) (ABIN358251)

Antigène

Voir toutes WEE1 Anticorps
WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivité

  • 82
  • 46
  • 33
  • 2
Humain

Hôte

  • 92
  • 5
  • 2
Lapin

Clonalité

  • 97
  • 2
Polyclonal

Conjugué

  • 44
  • 7
  • 7
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp WEE1 est non-conjugé

Application

  • 80
  • 45
  • 28
  • 28
  • 20
  • 14
  • 8
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Épitope

    • 19
    • 15
    • 10
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer123

    Specificité

    This antibody detects WEE1 pSer123.

    Purification

    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogène

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human WEE1

    Isotype

    Ig Fraction
  • Indications d'application

    ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100. Dot Blot: 1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Antigène

    WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

    Autre désignation

    WEE1 / Wee1-Like Protein Kinase

    Sujet

    This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.Synonyms: WEE1hu, Wee1A kinase

    Poids moléculaire

    71597 Da

    ID gène

    7465, 9606

    UniProt

    P30291

    Pathways

    Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase
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