PICK1 anticorps (C-Term)

N° du produit ABIN359172

Détail du produit anti-PICK1 anticorps

Antigène: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PICK1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts to PICK1 (PRKCABP).

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRKCABP.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PICK1

Antigène: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Autre désignation: PICK1 (PICK1 Produits)

Synonymes: anticorps PICK, anticorps PRKCABP, anticorps Prkcabp, anticorps prkcabp, anticorps MGC131295, anticorps PICK1, anticorps DKFZp470L2127, anticorps protein interacting with PRKCA 1, anticorps protein interacting with C kinase 1, anticorps protein interacting with PRKCA 1 L homeolog, anticorps PICK1, anticorps Pick1, anticorps pick1, anticorps pick1.L

Sujet: PDZ domain, but not the AH domain, of PICK1 interacts with the C termini of the GTP-bound forms of ADP-ribosylation factor-1 (ARF1) and ARF3. The interactions with ARF5 and ARF6 are weak, suggesting that the PICK1 interaction is specific for class I ARFs and that it may regulate Golgi-to-endoplasmic reticulum vesicle transport. The PDZ domain of rat Pick1 interacts with the last 10 amino acids of the short C-terminal alternative splice variants of AMPA receptor subunits. It has thus been proposed that E-S-V/I-K-I, a sequence found in these 10 amino acids, is a novel PDZ-binding motif. PRKCA phosphorylates Pick1 efficiently but binds Pick1 in both the phosphorylated and unphosphorylated states. Consistent with a neuronal role for PICK1, the mouse homolog interacts with mouse AMPA glutamate receptors and colocalizes at excitatory synapses in the brain. Metabotropic glutamate receptor-7 (mGluR7) localizes specifically to presynaptic active zones. The extreme C-terminal 3 amino acids of mGluR7 have been shown to interact with the PDZ domain of PICK1. Immunofluorescence microscopy demonstrated that both proteins are localized at excitatory synapses in hippocampal neurons, with clustering of mGluR7 at synapses requires PICK1 C-terminal PDZ-binding residues. Mutant mGluR7 lacking the PDZ-binding residues localized diffusely along axons rather than at the synapse, suggesting a role for Pick1 as a scaffolding molecule at presynaptic sites.Synonyms: PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

ID gène: 9463, 9606

UniProt: Q9NRD5