PICK1 anticorps (C-Term)

N° du produit ABIN391109

Détail du produit anti-PICK1 anticorps

Antigène: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Épitope: AA 286-316, C-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PICK1 est non-conjugé

Application: Western Blotting (WB)

Homologie: B, Pr, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This PICK1 (PRKCABP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-316 amino acids from the C-terminal region of human PICK1 (PRKCABP).

Clone: RB5475

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-PICK1 (ABIN391109)

Xue, Zhang, Chen, Lin, Shi: "PDZ protein mediated activity-dependent LTP/LTD developmental switch at rat retinocollicular synapses." dans: American journal of physiology. Cell physiology, Vol. 298, Issue 6, pp. C1572-82, (2010) (PubMed).

Détails sur PICK1

Antigène: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Autre désignation: PICK1 (PRKCABP) (PICK1 Produits)

Synonymes: anticorps PICK, anticorps PRKCABP, anticorps Prkcabp, anticorps prkcabp, anticorps MGC131295, anticorps PICK1, anticorps DKFZp470L2127, anticorps protein interacting with PRKCA 1, anticorps protein interacting with C kinase 1, anticorps protein interacting with PRKCA 1 L homeolog, anticorps PICK1, anticorps Pick1, anticorps pick1, anticorps pick1.L

Sujet: PDZ domain, but not the AH domain, of PICK1 interacts with the C termini of the GTP-bound forms of ADP-ribosylation factor-1 (ARF1) and ARF3. The interactions with ARF5 and ARF6 are weak, suggesting that the PICK1 interaction is specific for class I ARFs and that it may regulate Golgi-to-endoplasmic reticulum vesicle transport. The PDZ domain of rat Pick1 interacts with the last 10 amino acids of the short C-terminal alternative splice variants of AMPA receptor subunits. It has thus been proposed that E-S-V/I-K-I, a sequence found in these 10 amino acids, is a novel PDZ-binding motif. PRKCA phosphorylates Pick1 efficiently but binds Pick1 in both the phosphorylated and unphosphorylated states. Consistent with a neuronal role for PICK1, the mouse homolog interacts with mouse AMPA glutamate receptors and colocalizes at excitatory synapses in the brain. Metabotropic glutamate receptor-7 (mGluR7) localizes specifically to presynaptic active zones. The extreme C-terminal 3 amino acids of mGluR7 have been shown to interact with the PDZ domain of PICK1. Immunofluorescence microscopy demonstrated that both proteins are localized at excitatory synapses in hippocampal neurons, with clustering of mGluR7 at synapses requires PICK1 C-terminal PDZ-binding residues. Mutant mGluR7 lacking the PDZ-binding residues localized diffusely along axons rather than at the synapse, suggesting a role for Pick1 as a scaffolding molecule at presynaptic sites.

Poids moléculaire: 46600

ID gène: 9463

NCBI Accession: NP_001034672, NP_001034673, NP_036539

UniProt: Q9NRD5