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PGK1 anticorps (Middle Region)

Cet anticorps anti-PGK1 est un anticorps Lapin Polyclonal détectant PGK1 dans WB, IHC (p) et EIA. Adapté pour Humain et Souris.
N° du produit ABIN359199

Aperçu rapide pour PGK1 anticorps (Middle Region) (ABIN359199)

Antigène

Voir toutes PGK1 Anticorps
PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivité

  • 77
  • 42
  • 23
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Humain, Souris

Hôte

  • 67
  • 12
  • 1
  • 1
Lapin

Clonalité

  • 58
  • 20
Polyclonal

Conjugué

  • 52
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Cet anticorp PGK1 est non-conjugé

Application

  • 62
  • 35
  • 34
  • 18
  • 14
  • 12
  • 9
  • 5
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Épitope

    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region

    Specificité

    This antibody reacts to PGK1.

    Purification

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    Immunogène

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.

    Isotype

    Ig Fraction
  • Indications d'application

    ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry 1/50 - 1/100. Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Antigène

    PGK1 (Phosphoglycerate Kinase 1 (PGK1))

    Sujet

    Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.Synonyms: Cell migration-inducing gene 10 protein, PGKA, PRP 2, Primer recognition protein 2

    ID gène

    5230, 9606

    UniProt

    P00558

    Pathways

    Cellular Glucan Metabolic Process
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