GAPDHS anticorps

N° du produit ABIN361377

Cet anticorps anti-GAPDHS est un anticorps Souris Monoclonal détectant GAPDHS dans WB et IF. Adapté pour Porc.

Aperçu rapide pour GAPDHS anticorps (ABIN361377)

Antigène: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDHS est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1D4

Détail du produit anti-GAPDHS anticorps

Specificité: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Homologie: Avian, bovine

Purification: Total IgM fraction--concentrated culture supernatant

Immunogène: Purified porcine GAPDH

Isotype: IgM

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1,000 IF: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgM fraction + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur GAPDHS

Antigène: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Autre désignation: GAPDHS

Sujet: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Purification: Unpurified, concentrated culture supernatant.

Poids moléculaire: '38 kDa

ID gène: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process