CaMKII alpha/beta (pThr286) anticorps

N° du produit ABIN361458

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans WB. Adapté pour Rat. Ce Primary Antibody a été cité dans 11+ publications.

Aperçu rapide pour CaMKII alpha/beta (pThr286) anticorps (ABIN361458)

Antigène: CaMKII alpha/beta

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Épitope: pThr286

Specificité: Specific for the ~50k a-CaM Kinase II and the ~60k ß-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the (-phosphatase treatment.

Réactivité croisée: Souris, Rat (Rattus)

Homologie: human, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Référence

Lee, Shin, Zhong, Kim, Kim, Kim, Heo, Kim, Yeo, Kim, Liu: "Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes." dans: Nature communications, Vol. 9, Issue 1, pp. 3434, (2018) (PubMed).

Murakoshi, Shin, Parra-Bueno, Szatmari, Shibata, Yasuda: "Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor." dans: Neuron, Vol. 94, Issue 1, pp. 37-47.e5, (2017) (PubMed).

Bernard, Castano, Bayer, Benke: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." dans: Neuropharmacology, Vol. 84, pp. 1-12, (2014) (PubMed).

Boguslavsky, Chiu, Foley, Osorio-Fuentealba, Antonescu, Bayer, Bilan, Klip: "Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles." dans: Molecular biology of the cell, Vol. 23, Issue 20, pp. 4065-78, (2012) (PubMed).

Coultrap, Barcomb, Bayer: "A significant but rather mild contribution of T286 autophosphorylation to Ca2+/CaM-stimulated CaMKII activity." dans: PLoS ONE, Vol. 7, Issue 5, pp. e37176, (2012) (PubMed).

Kothmann, Trexler, Whitaker, Li, Massey, OBrien: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, (2012) (PubMed).

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." dans: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).

Ferrario, Loweth, Milovanovic, Ford, Galiñanes, Heng, Tseng, Wolf: "Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²?-permeable AMPA receptors during the incubation of cocaine craving." dans: Neuropharmacology, Vol. 61, Issue 7, pp. 1141-51, (2011) (PubMed).

Coultrap, Buard, Kulbe, DellAcqua, Bayer: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." dans: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, (2010) (PubMed).

Vest, OLeary, Coultrap, Kindy, Bayer: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." dans: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, (2010) (PubMed).

Townsend, Mehta, Selkoe: "Soluble Abeta inhibits specific signal transduction cascades common to the insulin receptor pathway." dans: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33305-12, (2007) (PubMed).

Détail du antigène

Antigène: CaMKII alpha/beta

Autre désignation: CAMK2A/B

Sujet: Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002). Anti-Phospho Thr286 CaM Kinase II Western blot of rat brain lysate showing specific immunolabeling of the ~50k (- and the ~60k (-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with (-Ptase.

Poids moléculaire: 50/60 kDa

ID gène: 25400

UniProt: P11275