CAMK2A anticorps (pThr286)

N° du produit ABIN361458

Cet anticorps anti-CAMK2A est un anticorps Lapin Polyclonal détectant CAMK2A dans WB, IHC et ELISA. Adapté pour Rat. Ce Primary Antibody a été cité dans 11+ publications.

Aperçu rapide pour CAMK2A anticorps (pThr286) (ABIN361458)

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CAMK2A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-CAMK2A anticorps

Épitope: pThr286

Specificité: Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Réactivité croisée: Humain, Souris, Lapin, Rat

Homologie: human, Xenopus

Attributs du produit: Our CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Stockage commentaire: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-CAMK2A (ABIN361458)

Lee, Shin, Zhong, Kim, Kim, Kim, Heo, Kim, Yeo, Kim, Liu: "Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes." dans: Nature communications, Vol. 9, Issue 1, pp. 3434, (2018) (PubMed).

Murakoshi, Shin, Parra-Bueno, Szatmari, Shibata, Yasuda: "Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor." dans: Neuron, Vol. 94, Issue 1, pp. 37-47.e5, (2017) (PubMed).

Bernard, Castano, Bayer, Benke: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." dans: Neuropharmacology, Vol. 84, pp. 1-12, (2014) (PubMed).

Boguslavsky, Chiu, Foley, Osorio-Fuentealba, Antonescu, Bayer, Bilan, Klip: "Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles." dans: Molecular biology of the cell, Vol. 23, Issue 20, pp. 4065-78, (2012) (PubMed).

Coultrap, Barcomb, Bayer: "A significant but rather mild contribution of T286 autophosphorylation to Ca2+/CaM-stimulated CaMKII activity." dans: PLoS ONE, Vol. 7, Issue 5, pp. e37176, (2012) (PubMed).

Kothmann, Trexler, Whitaker, Li, Massey, OBrien: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, (2012) (PubMed).

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." dans: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).

Ferrario, Loweth, Milovanovic, Ford, Galiñanes, Heng, Tseng, Wolf: "Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²?-permeable AMPA receptors during the incubation of cocaine craving." dans: Neuropharmacology, Vol. 61, Issue 7, pp. 1141-51, (2011) (PubMed).

Coultrap, Buard, Kulbe, DellAcqua, Bayer: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." dans: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, (2010) (PubMed).

Vest, OLeary, Coultrap, Kindy, Bayer: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." dans: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, (2010) (PubMed).

Townsend, Mehta, Selkoe: "Soluble Abeta inhibits specific signal transduction cascades common to the insulin receptor pathway." dans: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33305-12, (2007) (PubMed).

Détails sur CAMK2A

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Autre désignation: CaM Kinase II

Sujet:

Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Description Logic: Our CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody is produced . It detects CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: CAMK2A/B

Poids moléculaire: 50 kDa/60 kDa

ID gène: 25400

UniProt: P11275

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction