GABBR2 anticorps (pSer783)

N° du produit ABIN361471

Cet anticorps Lapin Polyclonal détecte spécifiquement GABBR2 dans WB et IF. Il présente une réactivité envers Rat et a été mentionné dans 1 publication.

Aperçu rapide pour GABBR2 anticorps (pSer783) (ABIN361471)

Antigène: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABBR2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-GABBR2 anticorps

Épitope: pSer783

Specificité: Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by (-phosphatase treatment.

Réactivité croisée: Souris, Rat (Rattus)

Homologie: bovine, canine, chicken, human, non-human primates, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IF: 1:500 (Kuramoto et al., 2007) Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-GABBR2 (ABIN361471)

Marron Fernandez de Velasco, Hearing, Xia, Victoria, Luján, Wickman: "Sex differences in GABABR-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex." dans: Neuropharmacology, Vol. 95, pp. 353-60, (2015) (PubMed).

Détails sur GABBR2

Antigène: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Autre désignation: GABBR2

Sujet: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007). Anti-Phospho-Ser783 GABAB R2 Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Poids moléculaire: '102 kDa

ID gène: 83633

UniProt: O88871

Pathways: cAMP Metabolic Process