Tryptophan Hydroxylase 1 anticorps (pSer260)

N° du produit ABIN361499

L’anticorps Lapin Polyclonal anti-Tryptophan Hydroxylase 1 a été validé pour WB. Il convient pour détecter Tryptophan Hydroxylase 1 dans des échantillons de Rat.

Aperçu rapide pour Tryptophan Hydroxylase 1 anticorps (pSer260) (ABIN361499)

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Tryptophan Hydroxylase 1 anticorps

Épitope: pSer260

Specificité: Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260.

Réactivité croisée: Humain, Rat (Rattus)

Homologie: bovine, canine, chicken, mouse, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur Tryptophan Hydroxylase 1

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Autre désignation: TPH1

Sujet: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000). Anti-Phospho Ser260 Tryptophan Hydroxylase Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.

Poids moléculaire: '55 kDa

ID gène: 24848

UniProt: P09810

Pathways: Feeding Behaviour