Tyrosine Hydroxylase anticorps (pSer31)

N° du produit ABIN361502

Détail du produit anti-Tyrosine Hydroxylase anticorps

Antigène: Tyrosine Hydroxylase (TH)

Épitope: pSer31

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tyrosine Hydroxylase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.

Réactivité croisée: Souris, Rat (Rattus)

Homologie: non-human primate

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IF (frozen sections, Witkovsky et al., 2000): 1:1000 IHC (frozen sections, Witkovsky et al., 2000): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-Tyrosine Hydroxylase (ABIN361502)

Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." dans: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." dans: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." dans: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." dans: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Détails sur Tyrosine Hydroxylase

Antigène: Tyrosine Hydroxylase (TH)

Autre désignation: Tyrosine Hydroxylase (TH Produits)

Sujet: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Anti-Phospho Ser31 Tyrosine Hydroxylase Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31.

Poids moléculaire: '60 kDa

ID gène: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour