CAMKII gamma anticorps

N° du produit ABIN361644

L’anticorps Souris Monoclonal anti-CAMKII gamma a été validé pour WB, IHC, ELISA, IP, IF, ICC et RIA. Il convient pour détecter CAMKII gamma dans des échantillons de Rat. Il y a 1 publication disponible.

Aperçu rapide pour CAMKII gamma anticorps (ABIN361644)

Antigène: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CAMKII gamma est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Clone: 6G9

Détail du produit anti-CAMKII gamma anticorps

Specificité: Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Partially purified rat CaMKII

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:10000)
• IHC (1:2000)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-CAMKII gamma (ABIN361644)

Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." dans: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, (2010) (PubMed).

Détails sur CAMKII gamma

Antigène: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Autre désignation: CaMKII

Sujet: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

ID gène: 12322

NCBI Accession: NP_033922

UniProt: P11798

Pathways: Signalisation WNT, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity