Calnexin anticorps (C-Term)

N° du produit ABIN361827

L’anticorps Lapin Polyclonal anti-Calnexin a été validé pour WB, IHC, IF et ICC. Il convient pour détecter Calnexin dans des échantillons de Chien. Il y a 6+ publications disponibles.

Aperçu rapide pour Calnexin anticorps (C-Term) (ABIN361827)

Antigène: Calnexin (CANX)

Reactivité: Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Calnexin anticorps

Épitope: C-Term

Specificité: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Singe, Souris, Porc, Caille, Lapin, Rat, Mouton, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogène: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN361826 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-Calnexin (ABIN361827)

Quintas, Pérez-Núñez, Sánchez, Nogal, Hentze, Castelló, Revilla: "Characterization of the African Swine Fever Virus Decapping Enzyme during Infection." dans: Journal of virology, Vol. 91, Issue 24, (2017) (PubMed).

Hernández-Tiedra, Fabriàs, Dávila, Salanueva, Casas, Montes, Antón, García-Taboada, Salazar-Roa, Lorente, Nylandsted, Armstrong, López-Valero, McKee, Serrano-Puebla, García-López, González-Martínez et al.: "Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. ..." dans: Autophagy, Vol. 12, Issue 11, pp. 2213-2229, (2017) (PubMed).

Gordo-Gilart, Hierro, Andueza, Muñoz-Bartolo, López, Díaz, Jara, Álvarez: "Heterozygous ABCB4 mutations in children with cholestatic liver disease." dans: Liver international : official journal of the International Association for the Study of the Liver, (2015) (PubMed).

Gordo-Gilart, Andueza, Hierro, Martínez-Fernández, DAgostino, Jara, Alvarez: "Functional analysis of ABCB4 mutations relates clinical outcomes of progressive familial intrahepatic cholestasis type 3 to the degree of MDR3 floppase activity." dans: Gut, Vol. 64, Issue 1, pp. 147-55, (2014) (PubMed).

Williams, Tulke, Ilegems, Berggren, Broberger: "Expression of nucleobindin 1 (NUCB1) in pancreatic islets and other endocrine tissues." dans: Cell and tissue research, Vol. 358, Issue 2, pp. 331-42, (2014) (PubMed).

Martín-Acebes, Merino-Ramos, Blázquez, Casas, Escribano-Romero, Sobrino, Saiz: "The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis." dans: Journal of virology, Vol. 88, Issue 20, pp. 12041-54, (2014) (PubMed).

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: Calnexin

Sujet: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

ID gène: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis