ERK1/2 anticorps (pThr202)

N° du produit ABIN362300

Cet anticorps Lapin Polyclonal détecte spécifiquement ERK1/2 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

Aperçu rapide pour ERK1/2 anticorps (pThr202) (ABIN362300)

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1/2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-ERK1/2 anticorps

Épitope: pThr202

Specificité: The antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at threonine 202.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pThr202 (F-L-T (p) -E-Y) derived from Human p44, 42 MAP Kinase. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-ERK1/2 (ABIN362300)

Han, Quirion, Zheng: "WITHDRAWN: Glutamate Attenuates IGF-1 Receptor Signaling via NR2B containing NMDA Receptors 3 and neuronal Nitric Oxide Synthase." dans: Biochemical and biophysical research communications, (2011) (PubMed).

Détails sur ERK1/2

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Autre désignation: p44/42 MAP Kinase

Sujet: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2).

Poids moléculaire: 44 kDa

NCBI Accession: NP_001035

UniProt: P27361