SMAD3 anticorps (pSer425)

N° du produit ABIN362420

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAD3 dans IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SMAD3 anticorps (pSer425) (ABIN362420)

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Immunohistochemistry (IHC)

Détail du produit anti-SMAD3 anticorps

Épitope: pSer425

Specificité: The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer425 (C-S-S-V-S (p) ) derived from Human Smad3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: Smad3

Sujet: Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Poids moléculaire: 52 kDa

NCBI Accession: NP_001138

UniProt: P84022

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy