SAPK, JNK (AA 181-185) anticorps

N° du produit ABIN362918

Détail du produit

Antigène: SAPK, JNK

Épitope: AA 181-185

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: The antibody detects endogenous level of total SAPK/JNK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Peptide sequence around AA 181-185 (M-M-T-P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Référence

Thévenin, Zony, Bahnson, Colman: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." dans: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).

Détail du antigène

Antigène: SAPK, JNK

Autre désignation: SAPK/JNK

Synonymes: anticorps JNK-55, anticorps JNK2, anticorps JNK2A, anticorps JNK2ALPHA, anticorps JNK2B, anticorps JNK2BETA, anticorps PRKM9, anticorps SAPK, anticorps SAPK1a, anticorps p54a, anticorps p54aSAPK, anticorps AI851083, anticorps Prkm9, anticorps mitogen-activated protein kinase 9, anticorps MAPK9, anticorps Mapk9

Sujet: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Poids moléculaire: 54 kDa

NCBI Accession: NP_001128

UniProt: P53779