MAP1LC3A anticorps (AA 50-150)

N° du produit ABIN363516

Cet anticorps anti-MAP1LC3A est un anticorps Lapin Polyclonal détectant MAP1LC3A dans WB, IF, IHC (p), ICC et IP. Adapté pour Humain, Souris, Rat et Poisson zèbre (Danio rerio).

Aperçu rapide pour MAP1LC3A anticorps (AA 50-150) (ABIN363516)

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP1LC3A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Détail du produit anti-MAP1LC3A anticorps

Épitope: AA 50-150

Specificité: Reacts with LC3 from human, mouse, rat and Zebrafish

Réactivité croisée: Boeuf (Vache)

Réactivité croisée (Details): Predicted to react with Xenopus and bovine based on 100 % sequence homology.

Purification: Antigen affinity purified

Immunogène: Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Détails sur MAP1LC3A

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Autre désignation: Light Chain 3 (LC3)

Sujet: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

ID gène: 84557

Pathways: Autophagy