TLR9 anticorps (AA 268-284) (FITC)

N° du produit ABIN364233

Cet anticorps anti-TLR9 est un anticorps Souris Monoclonal détectant TLR9 dans WB, IHC, FACS, ICC, IHC (p) et IHC (fro). Adapté pour Humain, Souris, Rat, Chien, Singe et Cheval.

Aperçu rapide pour TLR9 anticorps (AA 268-284) (FITC) (ABIN364233)

Antigène: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivité: Humain, Souris, Rat, Chien, Singe, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR9 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-TLR9 anticorps (FITC)

Épitope: AA 268-284

Marque: IHC-plus™

Specificité: Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, ICC, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry (Intracellular): 0.1-2 μg/10^6 cells, cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM Tris, 150 mM sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles. Sensitive to light.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Détails sur TLR9

Antigène: TLR9 (Toll-Like Receptor 9 (TLR9))

Autre désignation: TLR9

Sujet: Name/Gene ID: TLR9
Family: Toll-like Receptor

Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9

ID gène: 54106

UniProt: Q9NR96

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades